Professor Nikolaus Loening published an article, co-authored by three L&C BCMB majors.
Davis SM, Romig BL, Abe AA, Loening NM. An improved variant of tobacco etch virus (TEV) protease that does not need reducing agents. Protein Sci. 2025 Mar;34(3):e70049. doi: 10.1002/pro.70049. PMID: 39969093.
Tobacco etch virus (TEV) protease is widely used in biochemistry laboratories because of its ability to cut proteins at very specific locations, which is often a necessary step in generating protein samples for research. In this work, we introduce a new mutant version of TEV protease that is easier to make and that has enhanced stability and solubility, properties that will help scientists use this protease to further their research. In addition, this version of TEV protease does not require the presence of additional chemicals, called reducing agents, to maintain its activity.